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Individual ions are finally detected by single-ion counting (with silicon surface-barrier detectors, ionization chambers, and/or time-of-flight telescopes). Alvarez and Robert Cornog of the United States first used an accelerator as a mass spectrometer in 1939 when they employed a cyclotron to demonstrate that He was stable; from this observation they immediately and correctly concluded that the other mass-3 isotope tritium was radioactive. Muller at the Lawrence Berkeley Laboratory recognised that modern accelerators could accelerate radioactive particles to an energy where the background interferences could be separated using particle identification techniques. His paper was the direct inspiration for other groups using cyclotrons (G. Yiou, in France) and tandem linear accelerators (D.
In a second stage of mass spectrometer, the fragments from the molecules are separated from the ions of interest.